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KMID : 0861020110260030075
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Korea Journal of Herbology
2011 Volume.26 No. 3 p.75 ~ p.82
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Effect of Chungganhaeju-hwan in Ethanol-induced Neuronal Cell Damage
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Ju Mi-Sun
Kim Hyo-Geun
Cho Hae-Jeong
Sim Jae-Jong
Jeon Yong-Jun
Oh Myung-Sook
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Abstract
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Objectives: In this study, we evaluated the effect of Chungganhaeju-hwan(CGHJH) on hydrogen peroxide(H₂O₂)-induced and ethanol(EtOH)-induced neuronal damage in vitro and in vivo, respectively.
Methods:We carried out the anti-oxidant effects of CGHJH against hydrogen peroxide(H₂O₂)-induced toxicity in HT22 and PC12 cells using thiazolyl blue tetrazolium bromide. Then, to investigate the protective effect on CGHJH against EtOH-induced memory impairment and hippocampal cell damage in male ICR mice, we performed novel object recognition test(NORT), and analysed the brain tissues after immunohistochemistry and western blotting.
Results:CGHJH showed protective effect from H₂O₂-induced cell toxicity at doses of 1~100㎍/mL in both HT22 and PC12 cells. CGHJH had also recovery effect from EtOH-induced memory impairment in ICR mice from NORT and it protected hippocampal cells against EtOH toxicity in the result of cresyl violet and NeuN immunoreactivity.
Conclusion: These results demonstrate that CGHJH has protective effect in neuronal cells against H₂O₂ and EtOH toxicities and this effect could be a main role of recovery effect on EtOH-induced memory loss.
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KEYWORD
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Chungganhaeju-hwan, ethanol, hydrogen peroxide, memory loss, hippocampal cells
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